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| 名称： | anti- beta actin antibody |
|---|---|
| 货号： | FNab00869 |
| 适应种属： | 人源,大鼠,小鼠 |
| 规格： | 100ug(微克) |
| 状态： | 液体 |
| 纯化方法： | 免疫亲和纯化 |
| 纯度： | 经SDS-PAGE分析,纯度大于95% |
| 宿主： | 兔 |
| 亚型： | lgG |
| 存储： | 用添加了0.02%叠氮化钠和50%甘油的PBS溶解,PH值7.3,可在-20℃下保存12个月（避免反复冻融） |
| 免疫原： | actin, beta |
| 免疫原别称： | ACTB antibody, Actin antibody, actin antibody, beta antibody, Actin antibody, cytoplasmic 1 antibody, B actin antibody, Beta actin antibody, F actin antibody, β actin antibody |
| 分子量： | 42 kDa |
| 验证应用： | ELISA, IF, WB, IHC, IP |
| 建议稀释比例： | WB: 1:500-1:5000; IHC: 1:20-1:500; IF: 1:50-1:500;IP: 1:500-1:2000 |
| UniProt ID： | P60709 |
| 克隆性： | 多克隆 |
| 背景介绍： | Beta actin, also named as ACTB and F-Actin, belongs to the actin family. Actins are highly conserved globular proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells. At least six isoforms of actins are known in mammals and other vertebrates: alpha(ACTC1, cardiac muscle 1), alpha 1(ACTA1, skeletal muscle) and 2(ACTA2, aortic smooth muscle), beta(ACTB), gamma 1(ACTG1) and 2(ACTG2, enteric smooth muscle). Beta and gamma 1 are two non-muscle actin proteins. Most actins consist of 376aa, while ACTG2(rich in muscles) has 375aa and ACTG1(found in non-muscle cells) has only 374aa. Beta actin has been widely used as the internal control in RT-PCR and Western Blotting as a 42-kDa protein. However, the 41 kDa cleaved fragment of beta actin can be generated during apoptosis process. This antibody was generated against N-terminal region of human beta actin protein and can cross-react with other actins. |
| 图像： |  Immunohistochemistry of paraffin-embedded human heart using FNab00869(ACTB antibody) at dilution of 1:100。  Various lysates were subjected to SDS PAGE followed by western blot with FNab00869(beta actin Antibody) at dilution of 1:2000。 |
