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发布时间:2023-11-06 15:36   咨询量:
  • 英文名称: anti- LC3 antibody
  • 产品货号: FNab04717
  • 适应种属:人源,大鼠,小鼠
  • 宿主:兔
  • 亚型:lgG
  • 验证应用: ELISA, WB, IHC, IF, IP
  • 简介:菲恩生物抗LC3抗体经SDS-PAGE分析,纯度大于95%,采用免疫亲和纯化方法,为用户提供高亲和力、高特异性的优质抗体。
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    产品详情
    名称: anti- LC3 antibody
    货号: FNab04717
    适应种属:人源,大鼠,小鼠
    规格:100ug(微克)
    状态:液体
    纯化方法:免疫亲和纯化
    纯度:经DSD-PAGE分析,纯度大于95%
    宿主:
    亚型:lgG
    存储:用添加了0.02%叠氮化钠和50%甘油的PBS溶解,PH值7.3,可在-20℃下保存12个月(避免反复冻融)
    免疫原: microtubule-associated protein 1 light chain 3 beta
    免疫原别称: LC3 antibody, LC3B antibody, MAP1A/1BLC3 antibody, MAP1A/MAP1B LC3 B antibody, MAP1A/MAP1B light chain 3 B antibody, MAP1ALC3 antibody, MAP1LC3B antibody
    分子量: 16 kDa
    验证应用: ELISA, WB, IHC, IF, IP
    建议稀释比例: WB: 1:500-1:2000; IP: 1:500-1:2000 ; IHC: 1:20-1:200; IF: 1:20-1:200
    UniProt ID: Q9GZQ8
    克隆性:多克隆
    背景介绍:Map1LC3, also known as LC3, is the human homolog of yeast Apg8 and is involved in the formation of autophagosomal vacuoles, called autophagosomes. Three human Map1LC isoforms, MAP1LC3A, MAP1LC3B, and MAP1LC3C, undergo post-translational modifications during autophagy. And they differ in their post-translation modifications during autophagy. Map1LC3 also exists in two modified forms, an 18-kDa cytoplasmic form that was originally identified as a subunit of the microtubule-associated protein 1, and a 16-kDa form that is associated with the autophagosome membrane. This antibody can cross react with MAP1LC3A, MAP1LC3B, and MAP1LC3C.
    图像:LC3抗体
    Immunohistochemistry of paraffin-embedded human gliomas using FNab04717(LC3 antibody) at dilution of 1:50。

         anti- LC3 antibody
    IP Result of anti-LC3 (IP: FNab04717, 3ug; Detection: FNab04717 1:1000) with mouse brain tissue lysate 4000ug。


         anti- LC3 antibody
    Chloroquine treated HeLa cells were subjected to SDS PAGE followed by western blot with FNab04717(LC3 Antibody) at dilution of 1:1000。

    

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